Abstract 12914: The Effect of Isosorbidemononitrate on Myocardium in a Swine Model of Cardiac Arrest

Abstract

Introduction: To investigate the protective effect of isosorbidemononitrate (IM) on myocardial injury after the return of spontaneous circulation (ROSC) in a swine model of ventricular fibrillation cardiac arrest (VFCA). Methods: Ventricular fibrillation was induced and untreated for 8 minutes in twenty domestic pigs. Defibrillation was attempted after 2 minutes of CPR. The animals were randomized into two groups: IM group (n=10) and saline control group (SA group, n=10). IM (2μg/kg/min) or the same volume of saline was administered for 6 hrs post-resuscitation. Post resuscitation myocardial function was evaluated using pulse-induced contour cardiac output (PiCCO) at 1, 6 and 24 hrs after ROSC. Serum troponin I was tested using the enzyme-linked immunosorbent assay (ELISA) at the same time points during 24 hours post-resuscitation. Echocardiography and transmission electron microscopy were performed at 72 hrs after ROSC. The duration of survival was monitored in all animals. Results: Significantly greater ejection fraction (EF) and cardiac output (CO) in the IM group at 1, 6 and 24 hrs after ROSC (Fig.1). Troponin I in the IM group was significantly lower than that in the SA group at 6 and 24 hrs after ROSC. Better cardiac function (Table 1) and less ultrastructural deterioration were observed in the MI group at 72 hrs after resuscitation. However, there is no significant difference in the duration of survival between the two groups. Conclusions: In a swine model of cardiac arrest, isosorbidemononitrate attenuated post-resuscitation myocardial dysfunction.