Abstract
Abstract RNA is the intermediate link of central dogma. However, RNA is rarely used as a therapeutic target for its flexible structure and instability. CRISPR/Cas13a is reported as a powerful tool for RNA knockdown. The potential amplification of CRISPR/Cas13a in cancer cells could be further studied. Here we overexpressed the Cas13a protein by lenti-virus vectors in glioma and other cancer cells, and found out that crRNA-EGFP could induce the “collateral effect” after knocking down the target gene. EGFRvIII is a unique EGFR mutant subtype in glioma. CRISPR/Cas13a system could induce cell death of EGFRvIII overexpression glioma cells. Single cell and RNA-seq analysis confirmed the comprehensive RNA interference by CRISPR/Cas13a treatment. Furthermore, CRISPR/Cas13a system could inhibit the formation of glioma intracranial tumor in mice. Our results exhibit the comprehensive RNA interference of CRISPR/Cas13a system in cancer cells, and demonstrated the powerful tumor elimination potential of this system. Citation Format: Qixue Wang, Yunfei Wang, Yanli Tan, Chunsheng Kang. Comprehensive RNA interference induced by CRISPR/Cas13a gene editing system in human cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1246.
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