Abstract 12294: Gut Microbial Features Associated With Sedentary Time But Not Physical Activity: Results From the Hispanic Community Health Study / Study of Latinos (HCHS/SOL)

Abstract

Introduction: Modest experimental and human evidence implicate a role for physical activity or sedentary lifestyle in the perturbation of the gut microbiome, yet large human studies are lacking. Methods: This study included 1846 US Hispanic/Latino adults without diabetes aged 23-82 years (mean age 48 years, 49% women) with gut microbiome data by shotgun sequencing of stools and accelerometer-measured moderate-to-vigorous physical activity (MVPA) and sedentary time from the HCHS/SOL population based cohort (2014-2017). We examined associations of MVPA and sedentary time with gut microbial features - Bray-Curtis dissimilarity by PERMANOVA, and alpha diversity and centered log ratio transformed relative abundance of species and KEGG pathway modules by a linear regression accounting for the complex survey design. Results: Prolonged sedentary time was associated with higher Shannon index (0.03 per 1 hr of sedentary time, SE=0.02, P=0.077) and Bray-Curtis dissimilarity (R 2 =0.32%, P<0.001), adjusting for age and sex, but MVPA showed no association. Among 197 species with at least 0.01% average relative abundance, sedentary time was positively associated with 30 species including 8 Bacteroides species, Alistipes putredinis and finegoldii , and inversely with 19 species including 7 Prevotella species, Roseburia faecis , and Bifidobacterium adolescentis (FDR <.1, Figure), whereas no species was associated with MVPA. A KEGG pathway module analysis suggested elevated methane metabolism, bacterial secretion, and carbon fixation with higher sedentary time (P<.05) but none after FDR correction (all FDR>.1). Positive associations were observed between MVPA with D-glucuronate degradation and semi-phosphorylative Entner-Doudoroff pathway (gluconate → glycerate-3P, found in halophilic euryachaea and Clostridium species) (P<.05 but FDR>.1). Conclusions: We observed a perturbed overall gut microbiome and relative abundance of species with sedentary time.