Abstract
Abstract Androgen and the androgen receptor (AR) play important roles in prostate cancer development, and androgen ablation has been the main therapeutic option for the treatment of locally advanced or metastatic prostate cancer. It has been reported that androgen-dependent LNCaP prostate cancer cells undergo apoptosis upon phorbol ester 12-myristate 13-acetate (PMA) treatment. In this study, we investigated whether transcription factor sLZIP is able to protect LNCaP cells from PMA-induced apoptosis. Results from cell morphology, cell cycle and apoptosis assay showed that PMA-induced apoptosis of LNCaP cells was inhibited in cells transfected with sLZIP. We also examined the effect of sLZIP on PARP cleavage and caspase 3 activation. PMA induced PARP cleavage and capase 3 activation, however, cells transfected with sLZIP exhibited reduced cleavage of PARP and caspase 3 in a dose dependent manner. sLZIP induced phosphorylation of ERK and increased cyclin D3expression. OverexpressionofsLZIPattenuatedPMA-mediatedTNF-α induction in LNCaP cells. These results indicate that human sLZIP negatively regulates PMA-induced apoptosis of prostate cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1220.
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