Abstract 1196: FZD7 plays a critical role in triple negative breast cancer proliferation

Abstract

Abstract Breast cancer is genetically and clinically heterogeneous. Triple negative cancer is a subtype of breast cancer, most of which is associated with poor outcome and the lack of benefit from target therapy. A pathway analysis in a microarray study was performed using triple negative breast tumor cells compared with non-triple negative breast tumor cells. Overexpression among several Wnt pathway genes, such as FZD7, LRP6 and TCF7 has been observed in triple negative breast tumors. To validate their function, inhibition of FZD7 using FZD7-shRNA was carried out. Noticeably decreased cell proliferation, suppressed invasiveness and colony formation in triple negative MDA-MB-231 and BT-20 cells were observed. Mechanism study indicated that these effects occurred through silencing the canonical Wnt signaling pathway, as evidenced by loss of nuclear accumulation of β-catenin and decreased transcriptional activity of TCF7. Our finding suggests that FZD7 involved canonical Wnt signaling pathway is essential for tumorigenesis of triple negative breast tumor. Thus, FZD7 may be a biomarker and a potential therapeutic target for triple negative breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1196. doi:10.1158/1538-7445.AM2011-1196