Abstract 11447: Interaction Between Alcohol Habits and Gene Polymorphism of Ghrelin on the Risk of Essential Hypertension in Japanese Population

Abstract

Objectives: To evaluate the interactions between SNP of ghrelin gene( GHRL ) and alcohol habits on the risk of hypertension (HT:BP≥140/90) in Japanese. Methods: We analyzed 19036 Japanese subjects enrolled in the genome banking project at Jichi Medical University, including 8607 HT cases and 10429 non-HT controls.We divided the subjects into four groups according to gender and age (≤49 or ≥50).Alcohol habits were categorized to never, sometimes, and daily drinker for frequency of drinking. SNP rs696217(GG/GT/TT) of GHRL was genotyped by TaqMan method. Because the incidence of TT was low(<5%), GT and TT were combined together. The frequencies of genotypes, alcohol habits, and the combinations of both were compared between cases and controls by chi-squared test. The strength of the associations was estimated by logistic regression analysis. For the interaction, we assessed the joint ORs, which were calculated for each of six combined categories created by cross-classification. Non-risk genotypes as reference were different in M≤49(GG) and others(GT/TT). Results: (1)Genotype distributions significantly differed in F≤49. The frequencies of alcohol habits and combined categories significantly differed in all groups. (2) Compared to GG, the ORs (95%CI) of GT/TT were 1.25(1.0-1.55)* in M≤49. Compared to GT/TT, the ORs (95%CI) of GG were 1.57(1.20-2.06)* in F≤49, 1.01(0.90-1.13) in M≥50, and 1.04(0.93-1.16) in F≥50.(3) Compared to never drinkers, the ORs (95%CI) of daily drinkers were 1.77(1.32-2.37)* in M≤49, 1.26 (0.88-1.80) in F≤49, 1.64(1.44-1.87)* in M≥50, and 1.13(0.90-1.43) in F≥50. (4) Compared to never drinker with GG, the joint ORs (95%CI) of daily drinker with GG or GT/TT was 2.04(1.39-2.99)* and 2.34(1.55-3.54)* in M≤49. Compared to never drinker with GT/TT, the joint ORs (95%CI) of daily drinker with GT/TT or GG was 1.02(0.47-2.21) and 2.01(1.24-3.24)* in F≤49, 1.64(1.32-2.03)* and 1.70(1.40-2.08)* in M≥50, and 1.05(0.69-1.58) and 1.21(0.90-1.62) in F≥50 *(p<0.05). Conclusions: The interaction between GHRL rs696217 and alcohol habits had significant effects on HT risk in Japanese females≤49 years. The interactions between GHRL genotypes and alcohol habits may contribute to young-onset hypertension in Japanese females.