Abstract
Cruciferous vegetables are characterized by high amounts of glucosinolates (GLSs) that are hydrolysed to isothiocyanates (ITCs) and other phytochemicals. The aim of the study was to verify the effect of broccoli intake on plasma levels of carotenoids, vitamins and ITCs and on glutathione S-transferase (GST) activity. Twenty healthy subjects, characterized for GSTM1 and GSTT1 genotype, participated in a cross-over intervention study (broccoli diet versus cruciferous-free diet). Subjects consumed a daily portion of broccoli (10 days, 200 g) providing glucosinolates (200 µmol non-indolyl ITCs evaluated by cyclocondensation reaction after myrosinase treatment), vitamin C (about 100 mg) and carotenoids (about 5 mg lutein and β-carotene). An increase of folate, carotenoids and ITC plasma concentrations was found. The increase of plasma ITC concentration was independent of the GST genotype. Broccoli intervention did not affect plasma GST activity. Broccoli is a bioavailable source of diverse compounds whose effects on endogenous defence systems deserve further investigation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Food Sciences and Nutrition
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
