Abstract
Chloramphenicol entered cells of the alga Nitella clavata slowly and the concentration of antibiotic in the cell sap tended to equilibrate with that in the treating solution. The permeability constant was 1.5 × 10 −7 cm/sec. The rate of chloramphenicol uptake was influenced by concentration and temperature, but not by pH or 2,4-dinitrophenol. Normal and starved cells absorbed chloramphenicol at an equal rate, and treated cells lost the antibiotic they had absorbed at a rate comparable to the rate of uptake. The results appear consistent with the view that chloramphenicol permeated cells of the alga by simple diffusion.
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