Abstract

Abstract Root systems of potato plants (Solanum tuberosum L. var. Russett Burbank) were treated with 14CO2 and translocation was evaluated by autoradiography. Treatments of less than 1 hour showed little radioactivity in the leaves. Between 1 and 6 hours, there was a substantial increase in radioactivity in both roots and leaves. The 14CO2 was translocated to the leaves as 14CO2 dissolved in the xylem sap and as components fixed in the roots then transported. Plant tissues were analyzed spearately for radioactivity as extractable, basic (amino acids), neutral (sugars), insoluble (starch), and acidic (organic acids and sugar phosphates) fractions. Between 70 and 80% of the total extractable radioactivity was in the acidic fraction; of this, 96% was found in malic acid with the remainder containing other organic acids, amino acids, and sugars.

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