Abstract
Water-swellable hydrogel microspheres based on cross-linked polyacrylamide were used as solid supports in immunoassay formats. Capture antibody was covalently bound at the surfaces and the pore size of particles was such that these antibodies were excluded from the interiors. Upon contact with a solution containing immunological reactants, water molecules quickly penetrated the microspheres, causing them to swell, thereby concentrating analyte at the surface. Using swellable particles, the antigen capture rate during the first 5 min of incubation with antigen was two times that of antibody-coated 0.79 cm polystyrene beads. In addition, antibodies attached to lightly cross-linked swellable microparticles proved resistant to inactivation by ultrasonic energy, which can be used to accelerate immunological interactions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.