Abstract
SUMMARYIn three‐dimensional (3‐D) fluorescence images produced by a confocal scanning laser microscope (CSLM), the contribution of the deeper layers is attenuated due to absorption and scattering of both the excitation and the fluorescence light. Because of these effects a quantitative analysis of the images is not always possible without restoration. Both scattering and absorption are governed by an exponential decay law. Using only one (space‐dependent) extinction coefficient, the total attenuation process can be described. Given the extinction coefficient we calculate within a non‐uniform object the relative intensity of the excitation light at its deeper layers. We also give a method to estimate the extinction coefficients which are required to restore 3‐D images. An implementation of such a restoration filter is discussed and an example of a successful restoration is given.
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