Absorption and conversion of a single oral dose of beta-carotene in corn oil to vitamin A in Sprague-Dawley rats with low reserve of vitamin A.

Abstract

This study was carried out to determine how much of a single oral dose of beta-carotene in oil is absorbed and how much of the absorbed dose is converted to retinoids in rats having a vitamin A reserve at the lowest end of adequate status. Weanling rats raised on a vitamin A-deficient diet for four weeks were given a single oral dose of either corn oil or beta-carotene dissolved in corn oil (1.86 mumol). Serum, liver, and the entire digestive tract of the rats were analyzed for carotenoids and retinoids. Results showed that 4 hours after dosing, 1.64 mumol (88%) of the dose of beta-carotene was found intact, with 17.6% found in the stomach, 21% in the small intestine, and 49.3% in the large intestine. A total of 0.28 mumol of newly formed retinoids (expressed as retinyl palmitate) was present in serum, liver, and mucosa of small intestine. The results suggest that a single oral dose of beta-carotene might not be an effective way of raising vitamin A status in rats.