Abstract
We present a spectroscopic method for measuring the fluorescence quantum efficiency of fluorophores that does not require calibration standards. Photothermal Deflection Spectroscopy (PDS) is combined with conventional absorbance spectroscopy, and is extracted from fits over the spectral range of interest. This technique obviates the need for multiple measurements—either on calibration standards or as a function of concentration—and yields results of in a single measurement consistent with previously reported values. Five different organic dyes are tested whose absorbance/fluorescence profiles span the visible spectrum and whose known quantum efficiencies range from 0 to near 1. The accuracy of the results over significant ranges of wavelength and quantum yield demonstrate the robustness and efficacy of this method.
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