Abstract
Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored TLR9 gene polymorphism C296T/ Pro99Leu (rs5743844) in cervical cancer susceptibility among Indian women. Methods: The genotyping of TLR9 Pro99Leu polymorphism in 110 cervical cancer patients and 141 healthy controls was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequency detected in both cervical cancer and control populations was 1.0 (CC), 0.0 (CT) and 0.0 (TT); while the allele frequency was found to be 1.0 (C) and 0.0 (T). Conclusions: The present study demonstrates no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study subjects.
Highlights
Cervical cancer is the fourth-most common cancer among women globally and second leading cause of cancer-related deaths in Indian women[1]
Considering the fact that cervical cancer is largely caused by high-risk human papillomavirus (hrHPV) infection and Toll-like receptor 9 (TLR9) has the ability to respond to viral DNA, the present study was designed to elucidate the association of the TLR9 Pro99Leu polymorphism with cervical cancer
The preliminary data obtained from the present study does not suggest a role for the TLR9 Pro99Leu polymorphism in cervical cancer susceptibility
Summary
Cervical cancer is the fourth-most common cancer among women globally and second leading cause of cancer-related deaths in Indian women[1]. Persistent infection of high-risk human papillomavirus (hrHPV) is considered as the chief causative agent of cervical cancer, variations in host genetic make-up do influence the risk of acquiring HPV infection, and susceptibility to cervical carcinogenesis[2,3,4]. In this context, variations in Toll-like receptors (TLRs), that play a crucial role in activating immune response by identifying pathogenassociated molecular patterns, have drawn significant attention, as single nucleotide polymorphisms (SNPs) in TLR genes have been shown to alter susceptibility to many infections and human diseases including cancer[5,6,7,8]. Conclusions: The present study results demonstrate no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports worldwide minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study participants
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