Absence of tolerance to the aversive stimulus properties of ethanol following oral ethanol self-administration

Abstract

Depending on the situation, ethanol can serve as a reinforcer in one paradigm and an aversive stimulus in another. The relationships between the two stimuli are not clear, particularly the behavioural adaptation following chronic ethanol exposure. We report on two experiments using an oral-self administration (OSA) paradigm and a conditioned taste aversion (CTA) paradigm. Male Wistar rats were exposed to ethanol using either the OSA or the CTA paradigm, and the consequences were examined in the same groups of rats by performing the other corresponding experiment. Thus, sensitisation or tolerance to the respective stimulus properties of ethanol would be detectable. For OSA experiments, rats were presented, under a free-choice setting, tap water and an ascending series of ethanol concentrations (2–10%) for up to 4 days per concentration. The amounts of ethanol and water consumed in 23-h sessions were measured. For CTA, a two-bottle procedure was employed. Distinctively flavoured solutions (saccharin or saline) were paired with IP injections of either ethanol (1.5 g/kg) or saline (1 ml/kg). Tests for aversion were made after two pairings, when both solutions were presented simultaneously for 10 min. At low concentrations of ethanol, drinking solution consumption was high, decreasing gradually with increasing concentrations; however, daily intake of orally self-administered ethanol remained stable. No significant differences could be established between the two groups tested. Ethanol preference [EtOH/EtOH + H 2O] was attenuated in rats experienced with the CTA procedure before the OSA experiment. Injections of ethanol produced marked CTAs, even in rats that had consumed ethanol in the OSA experiment. The absence of tolerance to the aversive stimulus effects suggests that this stimulus property may not play a significant role in the consumption of ethanol.