Abstract
Heat waves are extreme thermal events whose frequency and intensity will increase with global warming. As metabolic responses to temperature are time-dependent, we explored the effects of an exposure to several heat waves on the mitochondrial metabolism of zebrafish Danio rerio. For this purpose, zebrafish were acclimated at 26 °C or 31 °C for 4 weeks and some fish acclimated at 26 °C underwent 2 types of heat waves: 2 periods of 5 days at 31 °C or 10 days at 31 °C. After this acclimation period, mitochondrial respiration of red muscle fibres was measured at 26 °C and 31 °C for each fish, with the phosphorylation (OXPHOS) and basal (LEAK) respirations obtained with activation of complex I, complex II or complexes I and II. The respiratory control ratio (RCR) and the mitochondrial aerobic scope (CAS) were also calculated at both temperatures after the activation of complexes I and II. Under our conditions, heat waves did not result in variations in any mitochondrial parameters, suggesting a high tolerance of zebrafish to environmental temperature fluctuations. However, an acute in vitro warming led to an increase in the LEAK respiration together with a higher temperature effect on complex II than complex I, inducing a decrease of mitochondrial efficiency to produce energy at high temperatures. Increased interindividual variability for some parameters at 26 °C or 31 °C also suggests that each individual has its own ability to cope with temperature fluctuations.
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More From: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
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