Abstract
Paraguay was among the 16 countries that reported zero indigenous malaria cases in 2014. A cross-sectional observational descriptive study was performed in 100 adults from Santa Teresa, Paraguay. Parasite detection was carried out using seminested multiplex polymerase chain reaction (PCR) and microscopy. Among the participants, 44% were female and 56% were male, and 89% had a malaria history. No parasites were detected with either of the methods. There were no asymptomatic cases in Santa Teresa, and this finding is very promising. A longitudinal study should be performed to confirm that there are no asymptomatic cases in this locality.
Highlights
Paraguay was among the 16 countries that reported zero indigenous malaria cases in 2014
Parasite detection was carried out using seminested multiplex polymerase chain reaction (PCR) and microscopy
Plasmodium parasites can be identified by microscopy, which is the gold standard method. They can be identified using seminested multiplex-polymerase chain reaction (SnM-PCR), which is a molecular diagnostic approach that has been shown to be useful for the detection of subpatent parasitemias
Summary
Paraguay was among the 16 countries that reported zero indigenous malaria cases in 2014. In the locality of Santa Teresa in the district of Raul Arsenio Oviedo of the department of Caaguazú, 134 cases of infection with P. vivax (30% of its population) were reported in 2007, just before the epidemiological silence started (unpublished data of the SENEPA). They can be identified using seminested multiplex-polymerase chain reaction (SnM-PCR), which is a molecular diagnostic approach that has been shown to be useful for the detection of subpatent parasitemias.
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