Absence of an early increase of phospholipid-phosphate turnover in mitogen-stimulated B lymphocytes

Abstract

An early increase in the phosphate incorporation in phosphatidylinositol (PI) was observed in CBA spleen suspensions only upon stimulation with the T cell mitogens phytohaemagglutinin and Concanavalin A. No specific increase in phosphatidylinositol phosphate turnover was found with lipopolysaccharide (LPS). In nude cells neither PHA nor LPS had any effect on the phosphate incorporation in PI. Corticosteroid-resistant thymocytes stimulated with pokeweed mitogen showed a specific increase in PI phosphate incorporation in contrast to spleen cells from neonatally thymectomized, ALS-treated mice. It is concluded that B cells do not show an early increase in PI phosphate turnover and that different lymphocyte populations may show variations in their metabolic response towards a mitogenic stimulus.