Abstract
A cell-free protein synthesis system was derived from brains of young (3 month) and old (>23 month) male Fischer-344 rats in order to examine brain protein synthesis in relation to age. The system was shown to be capable of reinitiating protein synthesis in vitro, and of synthesizing protein from exogenously added mRNA. Optimal ionic conditions for amino acid incorporation were 200 mM potassium ion and 5 mM magnesium ion, and amino acid incorporation depended on addition of ATP, GTP, and an energy-generating system (creatine phosphate and creatine phosphokinase). Amino acid incorporation was sensitive to the initiation inhibitors aurintricarboxylic acid and sodium fluoride. Optimal conditions were independent of the age of the rat from which the brain was taken. There was no statistically significant relation ( p>0.05) between capacity of amino acid incorporation and age. The aggregation state of brain polyribosomes also did not differ between young (3 month) and old (30 month) rats. The results suggest that overall brain protein synthesis capacity is age-invariant in the rat.
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