Abscisic acid priming confers salt tolerance in maize seedlings by modulating osmotic adjustment, bond energies, ROS homeostasis, and organic acid metabolism

Abstract

This study aimed at investigating the influence of exogenous abscisic acid (ABA) on salt homeostasis under 100 mM NaCl stress in maize (Zea mays L. cv. Kaveri 50) through 3 and 5 days of exposure. The ratio of Na+ to K+, hydrogen peroxide (H2O2) and superoxide (O2•‒) accumulation, electrolyte leakage were the major determinants for salt sensitivity. Pretreatment with ABA [ABA (+)] had altered the salt sensitivity of plants maximally through 5 days of treatment. Plants controlled well for endogenous ABA level (92% increase) and bond energy minimization of cell wall residues to support salt tolerance proportionately to ABA (+). Salt stress was mitigated through maintenance of relative water content (RWC) (16%), glycine betaine (GB) (26%), proline (28%) and proline biosynthesis enzyme (ΔP5CS) (26%) under the application of ABA (+). Minimization of lipid peroxides (6% decrease), carbonyl content (9% decrease), acid, alkaline phosphatase activities were more tolerated under 100 mM salinity at 5 days duration. Malate metabolism for salt tolerance was dependent on the activity of the malic enzyme, malate dehydrogenase through transcript abundance in real-time manner as a function of ABA (+). Establishment of oxidative stress through days under salinity recorded by NADPH-oxidase activity (39% increase) following ROS generation as detected in tissue specific level. The ABA (+) significantly altered redox homeostasis through ratio of AsA to DHA (21% increase), GSH to GSSG (12% increase) by dehydroascorbate reductase and glutathione reductase respectively, and other enzymes like guaiacol peroxidase, catalase, glutathione reductase activities. The ABA in priming was substantially explained in stress metabolism as biomarker for salinity stress with reference to maize.