Abstract
The fungus Ceratocystis coerulescens Bakshi (strain RWD 390) has been shown to produce the plant hormone, abscisic acid (ABA). The production of ABA in defined liquid medium during a culture period of 50 days was measured by gas‐liquid chromatography. A considerable accumulation of ABA occurred in the stationary phase. Maximum ABA contents were 3.5 ng ml−1 in culture media and 218 ng (g dry weight)−1 in mycelial extracts.The ABA‐metabolizing capability of the fungus was investigated. Dihydrophaseic acid, and phaseic acid, ABA metabolites in higher plants, were not present in cultures of Ceratocystis coerulescens. When [2‐14C]‐ABA was fed to the fungus, the formation of [2‐14C]‐2‐trans, 4‐trans‐ABA and a second metabolite, less polar than ABA, was observed. This suggests a different metabolic pathway of ABA in the fungus.
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