Abscisic acid-catabolizing bacteria: A useful tool for enhancing phytoremediation

Abstract

Bacteria-facilitated phytoextraction has been gaining recognition for the phytoremediation of heavy metal (HM)-contaminated soils. Nevertheless, it remains unclear whether catabolizing abscisic acid (ABA) in hyperaccumulating plants via rhizobacteria could facilitate HM phytoextraction. In this study, inoculation with the ABA-catabolizing bacterium, Rhodococcus qingshengii, increased HM (Cd, Zn, Pb, and Cu) concentrations in the shoots of hyperaccumulators Vetiveria zizanioides, Brassica juncea, Lolium perenne L., Solanum nigrum L., and Sedum alfredii Hance grown in mildly and severely contaminated soils by 28.8%–331.3%, 8.5%–393.4%, 21.2%–222.5%, 14.7%–115.5%, and 28.3%–174.2%, respectively, compared with non-inoculated plants. The fresh biomass of these hyperaccumulators was elevated by 16.5%–94.4%, compared to that of the bacteria-free control. Phytoremediation potential indices, including bioconcentration and translocation factors, also revealed that the bacteria markedly boosted the phytoextraction efficacy from soil. Furthermore, principal component analysis (PCA) suggested that the effects of bacteria on the concentrations of Cd and Zn in hyperaccumulators were significantly correlated with ABA metabolism, but not with Pb and Cu. Combined with the synergistic effects on plant biomass, the bacteria also improved the phytoextraction of Pb and Cu in hyperaccumulators. Overall, the application of microorganism-assisted remediation based on ABA-catabolizing bacteria might be an alternative strategy for enhancing phytoremediation efficiency in HM-contaminated soils.