Abortifacient response and plasma vasoconstrictive activity after feeding needles from ponderosa pine trees to cattle and sheep.

Abstract

Consumption of needles from Pinus ponderosa (PN) during late pregnancy causes cattle, but not sheep, to abort. This differential response may be caused by differences in ruminal microflora or postabsorptive metabolism. Pine needles were fed (2 kg.cow-1.d-1 or .4 kg.ewe-1.d-1) mixed with corn silage. In Exp. 1, cows were assigned at 250 d of pregnancy to feed treatments (T): 1) silage, 2) PN+silage, or 3) pretreated with sheep ruminal fluid and fed PN+silage. Interval to parturition was 34.3, 11.3, and 8.3 d for the T1, T2, and T3, respectively (T1 vs T2 + T3, P < .01; T2 vs T3, P > .5). Inoculation with sheep ruminal fluid did not alter activity of the abortifacient agent of PN. In Exp. 2, pregnant and nonpregnant ewes and cows were fed silage or PN mixed with silage, and plasma was analyzed for uterine vasoconstrictive activity in an in vitro placentome perfusion bioassay. Consumption of PN decreased interval to parturition in cattle (P < .01) but not in sheep (P > .5) and increased vasoconstrictive activity (P < .05) in plasma from nonpregnant and pregnant cows and ewes. The PN-fed ewes had a greater incidence of dead lambs at parturition (0/8 vs 5/8 for control vs PN-fed, P < .01). We conclude that pregnancy is not required for increased vasoconstrictive activity induced by pine needles, that sheep and cattle do not differ in ruminal metabolism of the abortifacient compounds in PN, and that species differences are subtle and due to postdigestive differences in response to the abortifacient agent.