Abstract
BackgroundHuman Ectopic Pregnancy (hEP) is the second most common cause of pregnancy-related deaths in the first trimester. Without timely detection, EPs can lead to an increased rate of infertility and an elevated risk for future tubal EPs. In addition, most studies in the field focus on the effect of the fallopian tube (maternal factors) and ignore epigenetic changes in genes and proteins of the embryo, which may also cause EPs. Therefore, the present study hypothesized that embryos also play an important role in the development of EP. The study also speculated that DNA methylation is associated with ectopic pregnancy. Consequently, the effects of DNA methylation on the occurrence and development of ectopic pregnancy were investigated. Moreover, genome-wide DNA methylation of chorionic tissue from ectopic and intrauterine pregnancies was detected using Illumina HumanMethylation450 arrays.ResultsForty-three hypermethylated genes involved in the regulation of adhesion as well as gene transcription and translation were identified. Furthermore, the PPI network showed that AMOTL1, SDR42E1, CAMTA1, PIP5K1C, KIAA1614, TSTD1 and DNER may play important roles in the occurrence and development of ectopic pregnancy. In addition, SDR42E1, CAMTA1 and TSTD1 displayed higher levels of methylation in ectopic pregnancy while PIP5K1C and DNER showed low degrees of methylation.ConclusionsThe study reveals that abnormal increase in methylation may be an early indicator or an inducer of ectopic pregnancy. In addition, AMOTL1, SDR42E1, CAMTA1, PIP5K1C, KIAA1614, TSTD1 and DNER might play important roles in the occurrence and development of ectopic pregnancy. However, the specific molecular mechanisms are still unclear and require further studies.
Highlights
Human ectopic pregnancy is the second most common cause of pregnancy-related deaths in the first trimester [1, 2], and approximately 98% of Ectopic pregnancy (EP) occur in the fallopian tube [3]
The study sought to identify novel candidate genes associated with EPs by comparing genome-wide DNA methylation using Illumina HumanMethylation450 arrays of chorionic tissues from ectopic and intrauterine pregnancies
Widespread DNA methylation changes are associated with EPs in chorionic tissues Differential DNA methylation analysis was performed on a total of 485,577 sites, using these Cytosine Guanine (CpG) to quantify the methylation profiles resulted in the EP in chorionic tissue from ectopic pregnancy and intrauterine pregnancy (Fig. 1A)
Summary
Human ectopic pregnancy (hEP) is the second most common cause of pregnancy-related deaths in the first trimester [1, 2], and approximately 98% of EPs occur in the fallopian tube [3]. Most studies focus on the effect of the fallopian tube (maternal factors) and ignore epigenetic changes in genes and proteins of the embryo, which may cause EPs. the present study hypothesized that embryos play an important role in the development of EPs. For example, the rapid replication and division of blastocyst cells and the disappearance of the premature zona pellucida may lead to implantation in the fallopian tube. EPs can lead to an increased rate of infertility and an elevated risk for future tubal EPs. In addition, most studies in the field focus on the effect of the fallopian tube (maternal factors) and ignore epigenetic changes in genes and proteins of the embryo, which may cause EPs. the present study hypothesized that embryos play an important role in the development of EP. Genome-wide DNA methylation of chorionic tissue from ectopic and intrauterine pregnancies was detected using Illumina HumanMethylation450 arrays
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
