Abstract
The DNA synthesized by isolated nuclei from normal HeLa cells, or from cells previously treated with mitomycin C were compared by DNA-DNA reassociation kinetics with DNA synthesized in vivo. DNA synthesized in vivo was found to be 31% reassociated at Cot 50 whereas DNA synthesized in vitro by isolated nuclei was 40% reassociated at this Cot value. Isolated nuclei, from cells whose cellular DNA synthesis had been completely inhibited by mitomycin, synthesized DNA which was unusually repetitious. In these nuclei, the proportion of DNA hybridized by Cot 50 ranged from 45% to 60% of the total DNA. The abnormal distribution of DNA sequences formed by isolated nuclei is discussed in relation to DNA synthesis.
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