Abnormal expression of bcl-2 gene family in development of Barrett＇ s esophagus

Abstract

Objective To detect the differential expression genes （DEGs） between Barrett＇s esophagus （BE） and normal esophagus with oligomicroarray, and to explore the target genes related to the development of BE. Methods The total RNAs of matched BE and normal esophagus mucosa from same patient were isolated with one step Trizol method. Matched RNAs were qualified with 10g/L agarose gel electrophorests. After tRNA purification, cRNAs were synthesized and labeled with fluorescence, which were then hybridized with Agilent oligomicroarray containing 30,968 probes. The fluorescence intensity features were detected by Agilent scanner and quantified by software Feature Extraction. Results On average, 2 biopsies by disposable jumbo biopsy forceps provided approximately 5 μg RNA required for microarray. The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality. Among 2-fold DEGs, there were 142 up-regulated genes and 284 down-regulated genes including 15 bcl-2 related genes such as bcl-2, MCL1, BAX, BIK and BCLAF1. Conclusion Microarray-based studies are feasible in endoscopieally obtained tissues. The development of BE is a complicated process involving multi-genes, in which abnormal expression of bcl-2 family related genes might be involved, but the exact mechanism needs further research. Key words: Barrett esophagus; Genes, bcl-2 ; Gene differential expression profile