Abstract
WCN 2013 No: 1235 Topic: 2 — Movement Disorders Ablation of rat substantia nigra is a good acute model of Parkinson's disease for cell transplantation therapy S.-I. Hirano, Y. Tokumoto, Y. Nishikawa. Physiology, Osaka Dental University, Osaka, Japan; Tissue Engineering & Cell Therapy, Institute of Biomedical Research & Innovation, Kobe, Japan; Biochemistry & Integrative Medical Biology, School of Medicine, Keio University, Tokyo, Japan A valuable rat model of Parkinson's disease (PD) to study cell therapy was established. Forty-five female rats were examined. Using a stereotactic technique, we created an acute model of PD by highfrequency electric ablation of bilateral substantia nigra pars compacta. The ablation probe used was a 100 mm-long rigid bipolar needle electrode (1.0 mm in diameter). The rats developed persistent symptoms of PD, especially akinesia and postural instability. We tried all sorts of surgical protocols. The size and shape of the lesion that were ablated in a typical setting were evaluated. Then we determined that the optimal surgical parameter to place a lesionwas 1 Wof power for 15 s. The longterm viability of transplanted neural stem/progenitor cells was also examined and confirmed to be viable for more than 60 days following surgery. As the animal model was designed to suit the study of cell transplantation therapy, it did not utilize neurotoxic chemicals to make lesions and could avoid the harmful effects of them. The benefits of our ablationmodel of PDwere the rapidity and uniformity of appearance of the characteristics of Parkinsonism. Therefore we can identify the onset of PD strictly. This feature is a unique merit that is not shared by the othermethods. The technique described heremay provide a good acute model of PD for the research of intracerebral cell transplantation. This model will contribute to the progress in the field of cell transplantation therapy. doi:10.1016/j.jns.2013.07.414 Abstract — WCN 2013 No: 1201 Topic: 2 — Movement Disorders Distribution of wild and mutant torsinA in living cells WCN 2013 No: 1201 Topic: 2 — Movement Disorders Distribution of wild and mutant torsinA in living cells I. Toyoshima, E. Abe, S. Kamada. Neurology, Akita National Hospital of NHO, Yurihonjo, Japan; Neurology, Akita University School of Medicine,
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