Abstract
The effect of abiotic factor (temperature, pH, dissolved oxygen, carbon dioxide) on the reproduction of snail L. acuminata fed to bait containing 40% and 80% of 24h LC50 eugenol have been reported in this paper. Feeding of baits containing sub-lethal concentration of eugenol caused a significant reduction in fecundity, hatchability and survival of young snails. Maximum (1644 egg/20 snails) and minimum (228 eggs/ 20 snails) fecundity was observed in month of May and December, respectively. There was a significant (P<0.05) change in the endogenous level of protein, amino acid, DNA and RNA in the ovotestis of L. acuminata. Treatment of 80 % of 24h LC50 with eugenol in bait caused maximum reduction in protein (52.12% of control), amino acid (11.89% of control), DNA (30.90% of control) and RNA (13.12% of control) level. Simultaneous, inhibition in acetylcholinesterase (AChE) activity in nervous tissue was also noted. Maximum inhibition in AChE activity (36.19% of control) was observed in snail exposed to 80 % of 24h LC50 of eugenol.
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