Abstract

To lower the risk of obesity, diabetes, and other related diseases, the WHO recommends that consumers reduce their consumption of sugars. Here, we propose a microbiological method to reduce the sugar content in red beet juice, while incurring only slight losses in the betalain content and maintaining the correct proportion of the other beet juice components. Several yeast strains with different metabolic activities were investigated for their ability to reduce the sugar content in red beet juice, which resulted in a decrease in the extract level corresponding to sugar content from 49.7% to 58.2%. This strategy was found to have the additional advantage of increasing the chemical and microbial stability of the red beet juice. Only slight losses of betalain pigments were noted, to final concentrations of 5.11% w/v and 2.56% w/v for the red and yellow fractions, respectively.

Highlights

  • In the last two decades, there has been increasing consumer interest in food safety and nutrition

  • There is a need for safe, biological methods to reduce the sugar content in red beet juice, while stabilizing the natural betalain ratio and improving the sensory and nutritional quality of the juice

  • To determine the effect of each of the tested yeasts on extract reduction and betalain content, the red beet juice before fermentation was sterilized at 121 ◦ C for 20 min

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Summary

Introduction

In the last two decades, there has been increasing consumer interest in food safety and nutrition. Various fermentation processes can be used to reduce the sugar content of red beet juice, as well as improve its nutritional value and stability. There is a need for safe, biological methods to reduce the sugar content in red beet juice, while stabilizing the natural betalain ratio and improving the sensory and nutritional quality of the juice. When yeast metabolizes a sugar under anaerobic conditions, ethanol and carbon dioxide are the main fermentation products. The purpose of this study was to evaluate the potential of conventional and nonconventional yeast strains with different sugar and enzymatic profiles to reduce the sugar content while maintaining the compactness and the proportion of betalain fractions in red beet juice. The following parameters were controlled: sugar content; extract content during fermentation; ethanol formation; changes in the concentration/proportion of betalain dyes

Red Beet Juice
Sugar Content
Yeast Strains
Assimilation Profiles
Enzymatic Profiles
Red Beet Juice Fermentation
Fermentation Efficiency
Betalain Content
Sugar Index
2.10. Statistics
Sugar Content in Red Beet Juice
Enzymatic Fingerprinting
Control of Betalains and Sugar Index
Conclusions
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