Ability of Different Adjuvants to Modulate the Allergic Response to Olive Pollen

Abstract

RationaleDifferent adjuvants for specific immunotherapy are available to enhance the immune response to the allergen. Conventionally, aluminum hydroxide is the most widely used adjuvant in vaccines formulations. A comparative study was carried out to characterize the effect of different other adjuvants on the systemic allergen-induced responses and airway reactivity in a murine model of allergic airway inflammation.MethodsFemale BALB/c mice were subcutaneously sensitized with a crude extract of olive pollen (Olea europaea). One week after last dose different groups were immunized with Olea either alone or in combination with aluminum hydroxide, calcium phosphate or bacterial derivates such as MPL-A® or ISS (Immune Stimulatory Sequences containing CpG motifs). Intranasal challenge with Olea extract was performed twice, pre and post-treatment. The Olea-specific immunoglobulin response in serum and the bronchial hypereactivity to methacholine were compared in all groups at several time-points.ResultsSensitization with Olea elicited a specific antibody IgG1 response in all the animals. Immunization enhanced the Olea-specific IgG1 levels in all groups. A significant IgG2a response was only observed in the mice injected with ISS. Airway reactivity to methacholine was only significantly reversed in the mice treated with bacterial-derived adjuvants.ConclusionsThe intrinsic natures of the adjuvants play a different role in immunization. Allergen extracts containing bacterial-derived adjuvants are better candidates for immunotherapy of allergic diseases. RationaleDifferent adjuvants for specific immunotherapy are available to enhance the immune response to the allergen. Conventionally, aluminum hydroxide is the most widely used adjuvant in vaccines formulations. A comparative study was carried out to characterize the effect of different other adjuvants on the systemic allergen-induced responses and airway reactivity in a murine model of allergic airway inflammation. Different adjuvants for specific immunotherapy are available to enhance the immune response to the allergen. Conventionally, aluminum hydroxide is the most widely used adjuvant in vaccines formulations. A comparative study was carried out to characterize the effect of different other adjuvants on the systemic allergen-induced responses and airway reactivity in a murine model of allergic airway inflammation. MethodsFemale BALB/c mice were subcutaneously sensitized with a crude extract of olive pollen (Olea europaea). One week after last dose different groups were immunized with Olea either alone or in combination with aluminum hydroxide, calcium phosphate or bacterial derivates such as MPL-A® or ISS (Immune Stimulatory Sequences containing CpG motifs). Intranasal challenge with Olea extract was performed twice, pre and post-treatment. The Olea-specific immunoglobulin response in serum and the bronchial hypereactivity to methacholine were compared in all groups at several time-points. Female BALB/c mice were subcutaneously sensitized with a crude extract of olive pollen (Olea europaea). One week after last dose different groups were immunized with Olea either alone or in combination with aluminum hydroxide, calcium phosphate or bacterial derivates such as MPL-A® or ISS (Immune Stimulatory Sequences containing CpG motifs). Intranasal challenge with Olea extract was performed twice, pre and post-treatment. The Olea-specific immunoglobulin response in serum and the bronchial hypereactivity to methacholine were compared in all groups at several time-points. ResultsSensitization with Olea elicited a specific antibody IgG1 response in all the animals. Immunization enhanced the Olea-specific IgG1 levels in all groups. A significant IgG2a response was only observed in the mice injected with ISS. Airway reactivity to methacholine was only significantly reversed in the mice treated with bacterial-derived adjuvants. Sensitization with Olea elicited a specific antibody IgG1 response in all the animals. Immunization enhanced the Olea-specific IgG1 levels in all groups. A significant IgG2a response was only observed in the mice injected with ISS. Airway reactivity to methacholine was only significantly reversed in the mice treated with bacterial-derived adjuvants. ConclusionsThe intrinsic natures of the adjuvants play a different role in immunization. Allergen extracts containing bacterial-derived adjuvants are better candidates for immunotherapy of allergic diseases. The intrinsic natures of the adjuvants play a different role in immunization. Allergen extracts containing bacterial-derived adjuvants are better candidates for immunotherapy of allergic diseases.