Abietane diterpenes from the cones of Abies numidica de Lannoy ex Carrière (Pinaceae) and in vitro evaluation of their antimicrobial properties

Maya Belhadj Mostefa,Amin Abedini,Laurence Voutquenne-Nazabadioko,Sophie C Gangloff,Ahmed Kabouche,Zahia Kabouche

doi:10.1080/14786419.2016.1190723

Abstract

Eight known abietane diterpenes (1–8) were isolated for the first time from Abies numidica cones (Pinaceae). The structures of all compounds were established by means of 1D and 2D NMR, and UV spectral analyses. The hydromethanolic extract of A. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. The best antimicrobial activity was found against gram-positive bacteria (MIC ≤ 0.3 mg/mL) Bacillus subtilis, Enterococcus faecalis ATCC 1034, Staphylococcus aureus 8325.4, S. aureus CIP 53.154, Micrococcus luteus and Listeria innocua and against Candida yeasts. The determination of MIC’s of isolated products showed a high activity of compounds 4 and 6 against S. aureus, L. innocua (MIC = 62.5 μg/mL) and E. faecalis (MIC = 125 μg/mL).

Highlights

IntroductionAbout 50 species are distributed in North Africa, Europe, North and Middle America and highlands of Asia (Zheng et al 1978)
Abies is an important genus of the Pinaceae family
The phytochemical composition and the in vivo antimicrobial activity of the cones of A. numidica

Summary

Introduction

About 50 species are distributed in North Africa, Europe, North and Middle America and highlands of Asia (Zheng et al 1978). Abies species have exhibited several activities, including antitumour (Kim et al 2004), anti-inflammatory, antihypertensive, anti-ulcerogenic (Singh et al 2000), antibacterial (Vishnoi et al 2007), antifungal (Aoyama et al 1992), antitussive (Nayak et al 2003), and central nervous system activities (Nayak et al 2004). The cones of Abies numidica de Lannoy ex Carrière were used in folk medicine against cold, indigestion, stomachache, pulmonary, vascular and venereal diseases (Fujita et al 1995). The phytochemical composition and the in vivo antimicrobial activity of the cones of A. numidica

Discussion

Conclusion