Abstract
Genetic analysis of random amplified polymorphic DNA (RAPD) markers can be complicated by the sensitivity of the RAPD-PCR procedure and the dominant nature of the markers. Using a unique system of six doubled haploid peach parents and their seven resulting F 1 hybrid populations, we found that 18% of the 50 scorable bands from 25 primers did not follow expected inheritance patterns. Bands present in parents were not transmitted to the progeny, or non-parental bands appeared in the progeny. Differences in RAPD patterns were also observed between haploids and spontaneous diploid bud sports on the same tree. Because peach is a long-lived clonally propagated crop, it is possible that somatic rearrangements occur which would lead to this unexpected inheritance of RAPD markers. Such anomalous patterns indicate that RAPD markers should be used cautiously in peach mapping and genetic diversity studies.
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