Abstract
Previous studies have shown that formaldehyde (FA) could cause immunotoxicity by changing the number of T lymphocytes and that cytokines play a pivotal role in the regulation of T lymphocytes. However, the previously used cytokine detection methods are difficult to use in the measurement of several cytokines in a small amount of sample for one test. Therefore, the cytometric bead array (CBA) technique was used. CBA showed better analytical efficiency and sensitivity than the previous methods. C57BL/6 mice were exposed to the control (normal saline), low FA concentration (0.5 mg/kg), and high FA concentration (2 mg/kg) for 1 week or 1 month. The contents of cytokines, including Th1-related cytokines (IL-2, IFN-γ, and tumor necrosis factor), Th2-related cytokines (IL-4, IL-6, and IL-10), and Th17-related cytokines (IL-17A), were measured by using the BD FACS Canto II Flow Cytometer and analyzed by FCAP ArrayTM Software. Th1/Th2/Th17-related cytokines showed a slightly decreasing trend after low FA exposure. Conversely, a significantly increasing trend was found after high FA exposure. Th1/Th2/Th17-related cytokines all serve important functions in the immune reactions in mice after FA exposure.
Highlights
Formaldehyde (FA) is commonly used in both industrial and residential environments.FA has widespread exposure and health effects
We aimed to investigate the production of cytokines in the serum of C57BL/6 mice after short-term FA exposure (1 week or 1 month)
We clustered the results of the cytokine assays into three groups according to the cytometric bead array (CBA) Mouse
Summary
Formaldehyde (FA) is commonly used in both industrial and residential environments. FA has widespread exposure and health effects. Studying the physiological changes of various systems after FA exposure is important. Apart from its contact irritation symptoms, affecting the skin, eyes, and the upper and lower respiratory tract, numerous studies have focused on the immunotoxicity and carcinogenicity of FA. Previous studies showed the association between FA exposure and asthma [1,2]. FA exposure may result in immunosuppression by decreasing the CD8+ effector memory T cells [3]. FA was classified as a carcinogen (Group 1) for both human and experimental animals, according to the International Agency for Research on Cancer (IARC) [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Environmental Research and Public Health
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
