Abstract
BackgroundLaryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis. Accumulating evidences have identified the important roles of long noncoding RNAs (lncRNAs) in the initiation and progression of various cancer types; however, the global lncRNAs expression profile for metastatic LSCC is limited.ResultsIn the present study, we screen expression profiles of lncRNAs in advanced LSCC patients with paired tumor tissues and corresponding normal tissues by microarrays. We identify numerous differentially expressed transcripts, and after the necessary verification of the transcripts expression in expanded samples, we experimentally validate the expression patterns of the remarkable low expressed gene, SSTR5, and its antisense lncRNA, SSTR5-AS1. Downregulation of SSTR5 is detected in LSCC tissues and laryngeal carcinoma cells. Aberrant DNA hypermethylation of the CpG sites clustered in the exon 1 and accumulation of inactive histone modifications at SSTR5 promoter region may be epigenetic mechanisms for its inactivation in LSCC. SSTR5-AS1 may play antitumor role in LSCC and may be regulated by the hypermethylation of the same CpG sites with SSTR5. SSTR5-AS1 inhibits laryngeal carcinoma cells proliferation, migration, and invasion. SSTR5-AS1 increases the enrichment of MLL3 and H3K4me3 at the promoter region of SSTR5 by interacting with MLL3 and further induces the transcription of SSTR5. Furthermore, SSTR5-AS1 interacts with and recruits TET1 to its target gene E-cadherin to activate its expression.ConclusionThese findings suggest that the identified lncRNAs and mRNAs may be potential biomarkers in metastatic LSCC, and SSTR5-AS1 may act as a tumor suppressor as well as a potential biomarker for antitumor therapy.
Highlights
Laryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis
Hierarchical cluster analyses indicated that the expression patterns in LSCC tissues were significantly different from those in corresponding normal tissues (Fig. 1B)
E-cadherin is the reported ten–eleven translocation (TET) target gene [22, 23], so we considered whether SSTR5-AS1 interacts with TET to regulate the expression of E-cadherin
Summary
Laryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis. Head and neck squamous cell carcinoma (HNSC) is the sixth most common type of cancer worldwide [1]. Laryngeal cancer is one of the most common head and neck cancers with well-defined risk factors such as tobacco abuse. Laryngeal squamous cell carcinoma (LSCC) is the predominant pathological subtype of laryngeal cancer. Despite the improvements in diagnosis and treatment, there is no significant improvement in the 5-year survival rate for LSCC in the past 30 years (from 59.6 to 66.8%) [4]. Identifying specific biomarkers to enable effective targeted therapy strategies is urgent for the improvement in diagnosis, treatment, and prognosis of LSCC
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