Aberrant expression of TNRC6a and miR-21 during myocardial infarction.

Abstract

In the present study, aberrant expression of trinucleotide repeat-containing gene 6a (TNRC6a) and miR-21 was noted and documented in rat myocardial infarction. Briefly, Sprague-Dawley rat model was used for the development of myocardial infarction. Experiments such as histological analysis were carried out to confirm the histopathology of the myocardial infarction. The expression profile of TNRC6a and miR-21 was identified by using quantitative real-time PCR. In addition, immunoblotting was performed to validate the expression profile of TNRC6a and phosphatase and tensin homolog (PTEN). The histological analysis confirmed the progress of myocardial infarction in rat model. As the disease progresses, the protein TNRC6a expresses abnormally which in turn up-regulates the miR-21 after 3rd and 5thweek of infarction. Interestingly, miR-21 binds with its specific target genes PTEN and thereby degrades the target mRNA; as a result, its expression was down-regulated progressively and paved the development of myocardial infarction. The present study concludes that the aberrant expression of TNRC6a and miR-21 was documented during myocardial infarction. These findings play an important role in the diagnosis as well as pave a way for the development of drug targets for treating myocardial infarction.