Aberrant expression of Tiel gene in venous valves in great saphenous varicose vein

Abstract

Objective To investigate the aberrant expression of Tiel gene in venous valves in great saphenous varicose vein,and its role in pathogenesis of varicose vein of lower extremity.Methods Varicose veins group ( 15 samples) and normal control group ( 11 samples) were set up.By using immunohistochemistry staining,the expression of CD31 and Tiel in the first valves in great saphenous veins was detected.Western blotting was used to check the expression of Tiel protein in venous valves.Results In normal control group valves,there was no difference between proximal and distal sides endothelium in expressing CD31 (P ＞ 0.05 ).The proximal side endothelium expressed Tie1 stronger than distal side at the basal part (P ＜ 0.05 ),but this difference was not found at valve cusp (P ＞ 0.05 ).In varicose veins group,besides morphological changes of valves,the proximal side endothelia expressed CD31 weaker than diatal side endothelia ( P ＜ 0.05 ),and expressed Tiel much weaker than diatal side endothelia ( P ＜0.01 ).The expression of Tiel protein was undetectable in venous valves.Conclusion The decreased expression of Tiel in proximal side of venous valves may play a role in the pathogenesis of primary lower extremity varicose veins. Key words: Tiel gene; Venous valve; Varicose vein