Aberrant expression of microRNAs induced by high glucose and the influence of miR-301a on the proliferation of prostatic epithelial cells

Abstract

Objective To study the aberrant expression of miRNAs implicated in the prostate under high-glucose treatment and to uncover the molecular impact of hyperglycemia exerted on the prostate.Methods A hyperglycemia rat model was induced by intraperitoneal injection of streptozotocin on male Sprague-Dawley rats.MicroRNA array was taken to detect the different expression of miRNAs in prostate tissues of rats,with quantitative real time polymerase chain reaction (qRT-PCR) adapted to confirm the alteration. Furthermore,these changes were also investigated in the human prostate epithelial cell line RWPE-1 in vitro.Methyl thiazol tetrazolium (MTT) assay was performed to evaluate the influence of high glucose (25-50 mmol/L) and/or miRNAs transfection on the proliferation of RWPE-1. Results Compared to the control, four miRNAs were significantly up-regulated (miR-186 240.5％, miR-301a 220.2％,miR-365 209.3％,miR-193 231.7％ ) in the hyperglycemia group ( P ＜0.05 ),while two miRNAs ( miR-434 29.8％,miR-361 38.6％ ) were obviously down-regulated ( P ＜ 0.05).These changes were consistent with the qRT-PCR results in RWPE-1 ceils cultured in high-glucose condition.MTT assay showed the cellular absorbance values (A values) of high-glucose groups and miR-30la-transfeeted group increased by 75％,97％ and 88％ respectively at the 48th hour,while 22％ for the control (P ＜0.05).Meanwhile,the A value of the 50 mmoL/L group with miR-301 a-inhibitor transfected was significantly less than that of the 50 mmol/L group ( P＜0.05 ).Conclusion Differential expression of miRNAs could be induced in prostate by high-glucose condition both in vitro and in vivo.Hyperglycemia and miR-301 a have positive effect on prostatic epithelium proliferation. Key words: High glucose; Prostate; MicroRNA; MiR-301 a; Proliferation