Abstract
ObjectiveTo observe the effects of WuziYanzong Pill (WYP) on Cox7a2 gene, Claudin-11 expression and to investigate extracellular signal-regulated kinase (ERK) phosphorylation in rat with kidney essence deficiency syndrome.MethodsFive groups of Sprague-Dawley male rats (5 in each group) were given vehicle, multiglycosides of Tripterygium Wilfordii Hook f (GTW) alone (20 mg/kg), or GTW followed by WYP (0.5, 1.0, or 2.0 g/kg daily) respectively for 30 days. Cox7a2 mRNA expression in testis was determined by real-time reverse transcriptase polymerase chain reaction. The extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation level and Claudin-11 expression were assessed by western blot analysis.ResultsGTW induced increased cox7a2 mRNA expression and an activation of ERKas well as a decline of Claudin-11 expression. Compared with GTW group, ERK phosphorylation level in high-dose WYP group decreased greatly with upregulated Claudin-11 expression. Furthermore, high-dose and mid-dose WYP down regulated cox7a2 mRNA levels.ConclusionsWYP protected the impaired spermatogenesis possibly through mediating mitochondrial energy metabolism and blood test is barrier function via ERK pathway.SupportSupported by National Natural Science Foundation of China (No. 81072809 and 81273767).
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