AB188. Vasohibin-1 and microvessel density predict prognosis in clear cell renal cell carcinoma

Abstract

ObjectiveTo investigate the expression of vasohibin-1(VASH-1) and microvessel density (MVD) marked with CD34 in clear cell renal cell carcinoma (ccRCC) and analyze their correlations with prognosis.MethodsFrom January 2006 to December 2008, the clinical and pathologic data of 79 patients with ccRCC were retrospectively analyzed. Expressions of VASH-1 and CD34 in their tissue samples were detected by immunohistochemical (IHC) staining. For VASH-1 assessment, the staining intensity of positive tumor cells were evaluated semi-quantitatively and measured by Average Optical Density (AOD) for further analysis. The microvessel density (MVD) was counted using Weidner’s method. Statistical analysis was performed using SPSS 19.0.ResultsThe AOD of VASH1 in the 79 cancer tissues was 0.0435±0.0178. The MVD value marked by CD34 in the 79 cancer tissues was (55±34) profiles/HPF. The expression of MVD and VASH1 significantly varied with tumor stages and necrosis presence (P<0.05). Assessed by Spearman’s correlation, the expression of VASH1 was negatively associated with MVD (r=−0.641, P<0.05). The overall survival (OS) and recurrence-free survival (RFS) in ccRCC patients with high VASH1 expression were significantly worse than those low VASH1 expression (P<0.05).The multivariate analysis showed that age, tumor stage and necrosis presence were significantly correlated to OS and RFS in ccRCC patients.ConclusionsVASH1 is potentially valuable in prognostic evaluation of ccRCC.