AB0870 PAIN AND FUNCTIONAL OUTCOME OF PERIPHERAL BLOOD DERIVED STEM CELLS IN PRIMARY OSTEOARTHRITIS OF KNEE AT LOW RESOURCES SETTING: A PHASE II PILOT RCT

Abstract

Background:Osteoarthritis (OA) of the knee is one of the main causes of musculoskeletal disability. Osteoarthritis is now often considered as organ failure. Because of limitations in the effectiveness of conventional management options, alternative possibilities such as cell based therapies are approaching into vogueObjectives:To assess the effectiveness of peripheral bold derived stem cells (PBSCs) therapy for primary OA knee.Methods:This phase-II pilot RCT was conducted after the IRB ethical clearance. Patients attending PM&R department of BSMMU having KL grade III osteoarthritis knee those fulfilled the selection criteria were considered as the sample. All respondents were divided to into two groups by using randomization technique. In control group, 15 respondents received standard care for Knee Osteoarthritis. In PBSCs group, 15 cases received with single dose autologous peripheral blood derived stem cells (PBSCs). Stem cells were harvested using G-CSF 30MU and CD34 stem cells were collected through apheresis. Quality was ensured measuring cell viability and surface antigen. All respondents were assessed before treatment and at week 4, week 12 and week 24 for pain reduction and for functional improvement using Visual Analogue Scale (VAS 0-10 cm) and validated Bengali WOMAC questionnaire. Joint sonography was done before treatment, at 12 week and 24 week after treatment commencement.Results:Female were 60% and 67% in control and PBSCs group respectively. The mean age was 56.3±6.7 and 55.8±7.3 year in control and PBSCs. After 12 weeks of stem cell therapy, pain reduction and functional significant improvement was observed in both groups. However, after 24 weeks further significant pain reduction, functional improvement was observed in PBSCs group. Moreover, the cartilage thickness measured by high frequency ultrasound and the mean cartilage thickness was increased by 0.14 mm from the baseline among PBSCs group.Conclusion:PBSCs is promising in patients with primary OA knee. Further large scale research is solicited.