AB0573 PERIPHERAL BLOOD B-CELLS SUBSETS IN PATIENTS WITH SJOGREN’S SYNDROME DEPENDING ON SOME IMMUNOLOGICAL PARAMETERS.

Abstract

ObjectivesTo examine В-cell subsets in peripheral blood of Sjogren`s syndrome (SjS) patients (pts) and to analyze the association between B-cell subsets and different clinical and immunological parameters.Methods67 active SjS pts (65F/2M); mean age 51±14,4 years; disease duration 60 (24-120) months were included. SjS was diagnosed based on the ACR-EULAR2016 criteria. Twenty healthy donors composed the control group. CD19+B cells, memory B-cells (CD19+CD27+), non-switched memory B-cells (CD19+IgD+CD27+), switched memory B-cells (CD19+IgD-CD27+), naïve (CD19+IgD+CD27-), double negative (CD19+IgD-CD27-), transitional (CD19+IgD+CD10+CD38+) B-cells, plasmablasts(CD19+СD38+++IgD-CD27+) were analyzed by multicolor flow cytometry using cytometer Navios (Beckman Coulter, USA).The nonparametric Mann-Whitney test, the Pearson`s x2 criterion, Spearman rank correlation coefficient were used for statistical analysis. Data were shown as median (Me) with an interquartile range of 25 - 75 percentile. The differences were considered statistically significant when p<0.05. Statistica 10 for Windows (StatSoft Inc., USA) package was used for statistical data processing.ResultsThe percentages/absolute numbers of plasmablasts, transitional B-cells, memory B-cells in SjS were significantly higher than in healthy donors, p<0.01 for all cases. At the same time percentages/absolute numbers of naïve cells in SjS were reduced compared to healthy donors (p=0.005, p=0.04). Shorter duration of SjS (less than 2 years) correlated with higher rates of naïve cells (p=0.02).ESSDAI score was ≥5 in 33 pts and <5 in 34 pts. We didn`t find any correlation between B cells subsets and disease activity. The presence of lymphoma in pts (n=5) had no impact on B-cells subsets as well. Significant changes were in B-cells subsets were found depending on immunological parameters. Pts with an isolated increase of antinuclear antibodies (ANA) (ANA positive, RF/aRo/aLa negative) had no plasmablasts in peripheral blood, level of plasmablasts in other pts was 1.5 (1-4.5)x103/µL, р=0,004. ARo-positive pts had higher amount of plasmablasts and transitional B-cells than aRo-negative pts (2 (1-5) and 1 (0-1) x103/µL, p= 0.004). Higher level of plasmablasts and transitional B-cells also correlated with decrease in C3 and/or C4 (p≤0.03) and increase in IgG (p<0.025).ConclusionAccording to our data elevated level of plasmablasts and transitional B-cells had positive correlation with some immunological diagnostic markers and immunological activity parameters of SjS but didn`t associate with ESSDAI.Disclosure of InterestsNone declared