Abstract
BackgroundThis study investigated the role of ZEB1 in vasculogenic mimicry (VM) formation and the interplay between VM and epithelial-mesenchymal transition (EMT).MethodsNinety-two prostate cancer tissue specimens were stained by CD34 and periodic acid Schiff. Then, we stained ZEB1 protein in the consecutive sections. Moreover, prostate cancer cells were subjected to ZEB1 knockdown using ZEB1 siRNA and then to 3D culture assay. EMT related maker was also evaluated.ResultsThe data showed that the presence of VM and high ZEB1 expression were associated with higher Gleason score, TNM stage, and lymph node and distant metastases. ZEB1 knockdown reduced VM formation and the expression of EMT-related in prostate cancer cells.ConclusionsIn the current study was the first to reveal that ZEB1 played an important role in VM formation in prostate cancer ex vivo and in vitro. Mechanistically, this process may have a relationship with EMT.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
