AB0066 EFFECT AND MECHANISM OF QINXITONG ON BIOLOGICAL BEHAVIOR OF SYNOVIAL FIBROBLASTS IN RHEUMATOID ARTHRITIS

Abstract

BackgroundChinese medicine has been used widely for the treatment of RA for a long history in China. The Fifth Hospital of Xi’an has treated patients of RA with QinXiTong(QXT) for 40 years which is mainly based on the water extract from Caulis Sinomenii(CS). However, the molecular mechanism of its anti-rheumatism effect remains unclear.ObjectivesTo investigate the effects of Qinxitong on proliferation, apoptosis, migration and invasion of synovial fibroblasts in rheumatoid arthritis and its mechanism.MethodsTaking rheumatoid arthritis synovial fibroblast cell line MH7A as the research object. The cells were divided into four groups according to the different dosage of Qinxitong intervention, namely control group, low dose group (QXT-20), medium dose group (QXT-50) and high dose group (QXT-100). CCK-8 assay was used to detect cell proliferation, flow cytometry was used to detect cell apoptosis, Transwell assay was used to detect cell migration and invasion, and Western blotting was used to detect total Erk protein (t-Erk) and phosphorylated Erk protein (p-Erk) expression levels.ResultsCCK-8 assay showed that Qinxitong could inhibit the proliferation of MH7A cells, compared with the control group, cell proliferation in QXT-50 and QXT-100 groups was significantly reduced (p<0.05) after 24h, 48, and 72h intervention, the effect of QXT-100 was even more significant (p<0.001); flow cytometry showed that QXT could promote apoptosis of MH7A cells, compared with the control group, the apoptosis rate of QXT-50 and QXT-100 groups was significantly increased (p<0.05); Transwell assay showed that QXT could inhibit the migration and invasion of MH7A cells, compared with the control group, the QXT-50 and QXT-100 groups showed significantly higher inhibition of migration and invasion of MH7A cells (p<0.05); Western blotting showed that QXT could reduced p-Erk expression level, compared with the control group, the decrease of p-Erk expression level in QXT-50 and QXT-100 groups was statistically significant (p<0.001).ConclusionQinxitong can inhibit the proliferation, promote apoptosis, inhibit migration and invasion of rheumatoid arthritis synovial fibroblasts by regulating Erk signaling pathway, providing scientific basis for clinical application of Qixintong.Disclosure of InterestsNone declared