AB0049B CHANGES OF INNATE LYMPHOID CELLS IN PERIPHERAL BLOOD OF PATIENTS WITH PRIMARY SJöGREN’S SYNDROME AND ITS CORRELATIONS WITH CLINICAL MARKERS

Abstract

Background: Innate Lymphoid Cells (ILCs) are a novel group of innate immune cells, according to the cytokine profile, they were divided into three major subsets: ILC1(Lin–IL7R+NKp46+ILCs), ILC2(Lin–IL7R+CRTH2+ILCs) and ILC3(Lin–IL7R+CRTH2–CD117+ILCs)[1]. Little research on ILC in the pathogenesis of Primary Sjogren’s Syndrome(primary SS) Objectives: Our purpose is to explore the function and role of ILC in the pathogenesis of Primary Sjogren’s Syndrome and its correlations with clinical markers. Methods: 20 patients with pSS and 15 age-matched healthy non-immune-related diseases controls were enrolled. The frequency of ILCs, B cells, CD4+T and CD8+T cells from PBMCs was detected by flow cytometry. Analysis the subsets of ILCs in each group which compared with B cells and T cell subsets respectively and correlation with clinical serologic markers. Analyze the levels of IL-4, IL-9, IL-33, IL-22 and IFN-γ in each group by ELISA. Results: Compared with the control group, the percentage of ILC was decreased significantly in primary SS(P 0.05). The frequency of ILCs in all patients positively correlated with antinuclear antibody titer (ANA(D)) (r=0.295, P=0.0133), moreover, the frequency of ILC2 in primary SS was positively correlated with B cells(r=0.3896;p 0.05). Conclusion: The frequency of ILCs is related to ANA(D) of primary SS patients and ILCs play a critical role in the pathogenesis of primary SS. Its function and mechanism are worth further exploration. Reference [1] Spits H., Artis D., Colonna M., et al. Innate lymphoid cells-a proposal for uniform nomenclature[J]. Nat Rev Immunol, 2013, 13(2): 145-149. doi: 10.1038/nri3365 Disclosure of Interests: None declared