Abstract
1) Treatment of formalin-treated A1 and A2 red cells with the enzyme preparation from Clostridium tertium A destroyed their A activities and consistently increased H activities. These enzyme-treated A1 and A2 red cells did not react with antiserum prepared by immunizying the rabbit with N-deacetylase-treated A substance.2) The enzyme preparation from Cl. tertium A released galactosamine, galactose and N-acetylglucosamine from both A1 and A2 red cells, but did not release any galactosamine from O red cells.3) The number of A antigen sites per red cell was estimated to be, on A1 cells, about 1.60×106, on A2 cells, about 0.40×106. The number of A sites on A2 cells was approximately one quarter of that on A1 cells.
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