Abstract
Artemisia annua is an important medicinal plant producing the majority of the antimalarial compound artemisinin. Jasmonates are potent inducers of artemisinin accumulation in Artemisisa annua plants. As the receptor of jasmonates, the F-box protein COI1 is critical to the JA signaling required for plant development, defense, and metabolic homeostasis. AaCOI1 from Artemisia annua, homologous to Arabidopsis AtCOI1, encodes a F-box protein located in the nuclei. Expressional profiles of the AaCOI1 in the root, stem, leaves, and inflorescence was investigated. The mRNA abundance of AaCOI1 was the highest in inflorescence, followed by in the leaves. Upon mechanical wounding or MeJA treatment, expression of AaCOI1 was upregulated after 6 h. When ectopically expressed, driven by the native promoter from Arabidopsis thaliana, AaCOI1 could partially complement the JA sensitivity and defense responses, but fully complemented the fertility, and the JA-induced anthocyanin accumulation in a coi1-16 loss-of-function mutant. Our study identifies the paralog of AtCOI1 in Artemisia annua, and revealed its implications in development, hormone signaling, defense, and metabolism. The results provide insight into JA perception in Artemisia annua, and pave the way for novel molecular breeding strategies in the canonical herbs to manipulate the anabolism of pharmaceutic compounds on the phytohormonal level.
Highlights
Jasmonates (JA) refer to a collection of bioactive oxylipins that can be perceived by plants and serve their functions as a fundamental phytohormone with critical roles in plant defense and development.In plants, JA is a family of derivatives from the 16- or 18-carbon tri-unsaturated fatty acids [1]
CORONATINE INSENSITIVE 1 (COI1) paralogs in Artemisia annua, Helianthus annuus, Oryza sativa, and Zea mays were identified
COI1 paralogs in Artemisia annua, Helianthus annuus, Oryza sativa, and Zea mays were identified by BLAST against the Arabidopsis thaliana COI1 protein
Summary
Jasmonates (JA) refer to a collection of bioactive oxylipins that can be perceived by plants and serve their functions as a fundamental phytohormone with critical roles in plant defense and development.In plants, JA is a family of derivatives from the 16- or 18-carbon tri-unsaturated fatty acids [1]. Jasmonates (JA) refer to a collection of bioactive oxylipins that can be perceived by plants and serve their functions as a fundamental phytohormone with critical roles in plant defense and development. Jasmonic acid (JA), methyl jasmonate (MeJA), and jasmonoyl-isoleucine (JA-Ile) are well characterized jasmonates, with JA-Ile being the most bioactive form [2]. The metabolic networks and signaling pathway of jasmonates have been a research hotspot over the past decade. JA biosynthesis to JA signaling has been well documented. LOXs, ALLENE OXIDE SYNTHASE (AOS), ALLENE OXIDE CYCLASE (AOC), and OXOPHYTODIENOIC ACID REDUCTAXSE 3 (OPR 3) are critical genes encoding enzymes required for JA biosynthesis. COI1, MAPKs, MYC2/3/4/5, JAZs, and ORA59 are reported to play essential roles in the canonical JA signaling pathway [3,4,5]
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