A59: Comparative characteristics of the microbiota and cytological patterns of the cervical canal (CC) according to quantitative polymerase chain reaction (PCR) and liquid-based cytology (LC)

Abstract

Background Women’s reproductive health is significantly determined by the relative constancy of the vaginal microbiota (Ravel et al., 2011). Change of its qualitative and quantitative composition is regarded as an edge with pathology of the condition with extremely diverse effects: from decreasing the quality of life to enhancing the carcinogenic risks induced by anaerobic bacteria metabolites (Mavzyutov et al., 2007). It was shown that vaginal microbiota changes typical for bacterial vaginosis (BV) are accompanied by similar changes in the CC microbiota (Smith et al., 2014) that is associated with an increased risk of HIV, herpes virus type 2, HPV, and neoplasia contamination (Borgdorff et al., 2014). Purpose of the study To identify the bacteria types that influence the most significant changes in cytological patterns of the CC in women with BV. Materials and methods To identify cytological characteristics of the inflammatory process in the CC associated with BV and quantitative species-specific changes in cervical microbiota, cervical scrapings of 122 women (aged 18–45) were studied with LC (Krjukova, 2008) and real-time PCR (“Femoflor 16”, Russia). Results Following the cytological characteristics of the cellular composition (abs.), presence of columnar epithelium, state of cell nuclei, nuclear size, chromatin state, number of nuclei in the cell (abs.Val.), existence and nature of nuclear changes, changes of the cell shape, vacuolization of cytoplasm, cytoplasm clearing, cell cytolysis, signs of keratinization, changes of cell size, increased nuclear-cytoplasmic ratio and other factors, the women were divided into 2 groups. The first group consisted of women with cervicitis without vaginosis, and the other group- with cervicitis associated with BV. Quantitative molecular genetic studies have shown that in the cervicitis associated with BV, significantly higher values were characterized by total bacterial mass and the scraping content of Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp., Sneathia spp./Leptotrihia spp./Fusobacterium spp., Megasphaera spp./Veillonella spp./Dialister spp., Mobiluncus spp./Corynebacterium spp., Lachnobacterium spp./Clostridium spp., Peptostreptococcus spp., Atopobium vaginae and Ureaplasma spp. However, the concentration of Enterobacteriaceae and Mycoplasma hominis in cervicitis not accompanied and not associated with BV was not significantly different. According to LC, in cervicitis associated with BV, the test material contained statistically significant (p Conclusion 1. Cytological pattern of inflammation during BV differs from the pattern of cervical inflammation not associated with BV; 2. Quantitative changes of the CC microbiota in BV detected by PCR are correlated with the cytological features of the inflammation pattern; 3. The influence of inflammation caused by quantitative changes in the CC microbiota on the increased risk of neoplastic processes is not excluded.