A312 マイクロ流路内における疎水性粒子の付着パターン解析

Abstract

Microfluidic devices for cell culture have recently been fabricated for perfusion culture, which can provide more likely in vivo environments for cells by continuously supplying nutrition and oxygen, and removing wastes. To design integrated cell culture microfluidic devices with higher density and larger numbers of cells, a fundamental understanding of relationship between cell adhesions and fluid flow in the channel is necessary Eight types of obstacles were made in centre of the PDMS (polydimetylsyloxane) microchannel, and hydrophobic beads as mimic of living cells were introduced. We found that beads are mostly attached to certain positions of obstacles and microchannel walls, even in the high velocity gradient area. The surface of PDMS microchannel is hydrophobic, and the strong hydrophobic beads are more easily attached to the microchannel wall than that of weak hydrophobic beads. The beads are easily sedimented in the channels with square and circular obstacles, and sometimes finally blocked the fluid flow. Establishment of the evaluation method by the beads would be broadened to integrated cell devices.