Abstract
The synthesis and isolation in purified form of [A-D-Asn21] sheep insulin is described. For this purpose [D-Asn21] A chain S-sulphonate was synthesized by the fragment condensation approach and isolated in a purified form. Conversion of the latter into its thiol form and combination with the S-sulphonated B chain of bovine (sheep) insulin produced the [A-D-Asn21] sheep insulin, isolated by chromatography on a carboxymethylcellulose column with an exponential sodium chloride gradient. The [A-D-Asn21] insulin shows potencies (I.U. mg–1) of 8 when assayed by the mouse convulsion method and of 4 by the radioimmunoassay method (cf. 23–25 for the natural hormone).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Chemical Society, Perkin Transactions 1
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
