Abstract
The present study aimed to analyze sera proteins in females with cervical intraepithelial neoplasia, grade III (CIN III) and in healthy control females, in order to identify a potential biomarker which detects lesions that have a greater probability of cervical transformation. The present study investigated five sera samples from females who were Human Papilloma Virus (HPV) 16+ and who had been histopathologically diagnosed with CIN III, as well as five sera samples from healthy control females who were HPV-negative. Protein separation was performed using two-dimensional (2D) gel electrophoresis and the proteins were stained with Colloidal Coommassie Blue. Quantitative analysis was performed using ImageMaster 2D Platinum 6.0 software. Peptide sequence identification was performed using a nano-LC ESIMS/MS system. The proteins with the highest Mascot score were validated using western blot analysis in an additional 55 sera samples from the control and CIN III groups. The eight highest score spots that were found to be overexpressed in the CIN III sera group were identified as α-1-B glycoprotein (A1BG), complement component 3 (C3), a pro-apolipoprotein, two apolipoproteins and three haptoglobins. Only A1BG and C3 were validated using western blot analysis, and the bands were compared between the two groups using densitometry analysis. The relative density of the bands of A1BG and C3 was found to be greater in all of the serum samples from the females with CIN III, compared with those of the individuals in the control group. In summary, the present study identified two proteins whose expression was elevated in females with CIN III, suggesting that they could be used as biomarkers for CIN III. However, further investigations are required in order to assess the expression of A1BG and C3 in different pre-malignant lesions.
Highlights
Cervical cancer is the third most common type of cancer in the female population worldwide
Cervical cancer develops due to persistent infection with the oncogenic Human Papilloma Virus (HPV), which progresses into an intraepithelial lesion, into invasive cervical cancer
Serum protein profiles were analyzed in samples from females in the control group (n=5) and those diagnosed with cervical intraepithelial neoplasia (CIN) III through histopathological analysis (n=5)
Summary
Cervical cancer is the third most common type of cancer in the female population worldwide. Cervical cancer is considered to be the seventh most common type of cancer, with 530,232 cases reported in 2008. More than 85% of cases occur in developing countries [1]. The World Health Organization reports 33,000 new cases per year [2,3]. In Mexico, cervical cancer is the second most common cause of cancer‐associated mortality in females, and there is a population of 40.06 million 15‐year old females who are at risk of developing cervical cancer. It is estimated that each year, 13,960 females are diagnosed with cervical cancer and that 4,476 succumb due to the disease [4]. Cervical cancer develops due to persistent infection with the oncogenic Human Papilloma Virus (HPV), which progresses into an intraepithelial lesion, into invasive cervical cancer
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